Qian Lai followed Xiao Han into the laboratory. At this moment, the atmosphere inside became strange.

John had gotten to know most of the people in his team at the last dance. Everyone who saw her smiled and waved.

However, apart from the tall, thin man with gray hair standing at the front, the other researchers from this country all had cold and stern faces, even showing disdain.

"Xiao, let me introduce you. This is Leng Hengzhi, a senior researcher at the institute. Behind him is his assistant, Xiao Fan..."

Leng Hengzhi?

Where have I heard this name before?

Before Qian Lai could even recall what was happening, he heard Leng Hengzhi's assistant, Xiao Fan, say provocatively, "May I ask which university you're from? Where do you conduct your research? How come you can offer a solution to our problem?"

"I never went to university."

As soon as Qian Laiyu uttered those words, the researchers all showed expressions of surprise.

"Are you here to cause trouble?"

"I knew it. He looks like he's not even twenty years old yet, and he dares to ask two experts for advice. He really doesn't know his own limitations."

"We're a rigorous research institution here. You haven't even been to university, do you know what genes or sequencing are? And you dare to give us pointers?"

"That's incredibly overconfident."

Xiao Han translated all the arguments and gave them to John.

Before Qian Lai could even object, John was the first to disagree.

"Xiao's professional skills and theoretical level are in no way inferior to some of the people present here."

We cannot judge a person's level of knowledge based on their academic qualifications. My father never attended a so-called middle school or university.

However, this did not prevent him from becoming a scientist.

I hope everyone will not look at my friends with prejudice.

Leng Hengzhi smiled slightly, but his eyes were full of meticulous inquiry: "I trust Dr. Brown's judgment. Since he thinks so highly of you, I'd also like to hear your opinion, young lady."

Qian Lai smiled and said gently, "I only overheard a few words outside the door. It seems that the two experts have different opinions on how to accurately measure the differences in the expression level of target genes."

Both sides nodded in agreement.

"Dr. Brown proposed using the NBlot method, which is highly accurate and can perform precise analysis of different strains in all dimensions, both horizontally and vertically. However, for China, it may be impossible to implement in the short term due to funding constraints and various strict regulations."

Traditional methods are inadequate for accurately 'weighing' small, low-yield strains. So, could we utilize DNA polymerases from molecular biology and specific primers for the target gene to specifically 'replicate and amplify' only the desired gene?

After 2-3 manual 'replications,' how can we compare the high-expression strains with the amplified low-expression strains using a unified polymerase detection method?

Qian Lai simplified the fundamental theory of qPCR methods in later generations into a practical method that is currently applicable, and then explained it to us.

The two experts listened very attentively, and after a moment of deep thought, they both looked at her, awaiting her conclusion.

"I recently came across an article in the Capital Science and Technology Monthly that outlined the concept of internal reference normalization. It should perfectly improve accuracy and comparability. It is closer to the molecular essence than traditional methods and is significantly more reliable. It is more practical and resource-efficient than NBlot."

Qian Lai knew that standardizing internal reference materials was the most reasonable and acceptable transitional approach to solving the current problem, and it was the most acceptable approach to both expert groups.

"You mean, to calculate an accurate result using a normalization method?"

A glint of starlight flashed in Leng Hengzhi's eyes.

Qian Lai nodded and continued, "In this way, we only need to compare the ratio of the strains' 'true contribution value'! This also greatly saves manpower and time costs."

Leng Hengzhi rested his chin on his hand, pondered for a long time, then turned to the team members who still looked bewildered and instructed them: "Go and find the article about 'internal reference standardization' in the Capital Science and Technology Monthly that this girl mentioned, and everyone should study it as soon as possible."

Then he looked at John and asked, "Dr. Brown, do you think this girl's method is feasible?"

John spread his arms and shrugged. "This is probably the best compromise you can accept right now, and one that we can trust. I told you, this girl is no ordinary person, right? Cold."

Hearing both sides' assessments, Qian Lai finally breathed a sigh of relief.

It's important to understand that trying to explain an existing scientific method using basic principles, while also relying on their known theoretical foundations, is as difficult as proving "my father is my father."

Fortunately, both of them are intelligent people who are not only rigorous in their approach to scientific research but also very tolerant.

"Young lady, I'm very curious. You didn't go to university, so how do you know such cutting-edge theoretical knowledge? And how are you able to integrate it and apply it in practice?"

A smile finally appeared on Leng Hengzhi's face.

"Because I have a good teacher who taught me how to learn on my own. She said, 'A thousand days of passive learning in the classroom is not as good as one hour of active exploration.'"

"oh?"

Upon hearing this, Leng Hengzhi's expression turned serious.

"Did your teacher say anything else? 'Actively exploring for a time requires persistent and years of effort'!"

Qian Lai and Leng Hengzhi read the second half of the sentence aloud in unison. The researchers looked at each other even more bewildered, completely clueless.

"Haha, so the child my junior sister mentioned in her letter was you? I was wondering why this child hadn't come to see me yet. Turns out, you wanted to amaze everyone before you could appear. Hahaha. Good child."

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