After handing over the affairs of Twilight Town to others, Todd buried himself in the laboratory building again.
Before we officially begin building the "Inheritors" army, there is one thing that needs to be confirmed first.
This is the human body's tolerance to Sutherland bacteria. In other words, if a large amount of Sutherland bacteria were injected into a normal human body, would it cause adverse reactions?
The principle behind this issue is that overuse of anything can have negative effects, especially of something as delicate and demanding as the human body. For example, overuse of ergotamine, triptan, and opioids can lead to varying degrees of headaches, muscle spasms, hallucinogenic visual disturbances, and addiction. Similarly, overuse of antibiotics can weaken the immune system and increase antibiotic resistance in bacteria, potentially leading to the emergence of superbugs.
Todd knows that only three people have been artificially injected with Sutherland bacteria: himself, Hudgens, and Edgar. As of today, none of them have experienced any adverse reactions. However, the amount of Sutherland bacteria injected into them was not large; Todd injected approximately 20 milliliters of the bacterial solution, while Hudgens and Edgar each injected less than 15 milliliters.
So, the first thing to calculate is the number of bacteria in the bacterial solution.
To determine the number of bacterial colonies, the colony forming units (CFU) method is used to detect *Sutherland bacteria*. The procedure involves serially diluting a 1 ml bacterial sample obtained from a volcanic environment culture flask with sterile physiological saline, obtaining appropriate dilutions (typically three dilutions), and then inoculating these dilutions onto plates using the spread plate method. After a certain incubation period, the colonies on the plates are directly counted. This method can determine the number of currently culturable viable microorganisms contained in a *Sutherland bacteria* sample; therefore, it is also known in microbiology as the "viable cell count method."
Todd ultimately determined that the unit density of the 'Sutherland bacteria' obtained in the volcanic environment culture flasks was approximately 170 cfu/ml (note that this refers to the number of colonies, not the number of bacteria). This translates to Todd injecting 3400 units (170 × 20) of 'Sutherland bacteria' colonies, while Hudgens and Edgar injected 2550 units (170 × 15) of colonies.
So here's the question.
Dig... what is the maximum number of Sutherland colonies the human body can hold?
Todd designed a drug tolerance experiment. The general idea was as follows: Sutherland bacteria were injected into mice, and the maximum drug tolerance of the subjects was observed. Then, based on the tolerance ratio between humans and animals, the bacterial tolerance in humans could be calculated.
What exactly is the tolerance ratio?
Humans and animals have vastly different tolerances to the same drug. Generally speaking, animals have a higher tolerance than humans; that is, the dosage per unit body weight is often several times, or even dozens of times, higher in animals than in humans. Therefore, the conversion of human dosage to animal dosage is generally as follows: assuming a human dosage of 1 unit, the dosage for mice and rats is 25-50 times, for rabbits and guinea pigs it is 15-20 times, and for dogs and cats it is 5-10 times.
The experiment officially began, and Todd selected five mice of similar age and weighing approximately 20 grams each. A 170 cfu/ml Sutherland bacterial solution was diluted tenfold to 17 cfu/ml, and the mice were injected once daily with a dose of 1 ml.
On day 9, after being injected with the bacterial solution 9 times (totaling 9 ml, 153 Sutherland colonies), the mice developed anorexia, vomiting, and low activity.
On day 11, after 11 injections of bacterial solution (totaling 10 ml, 187 Sutherland colonies), the mice developed epilepsy and convulsions.
On day 15, after 15 injections of bacterial solution (totaling 15 ml, 255 Sutherland colonies), the first mouse died 7 hours later and the second died 11 hours later.
After autopsy and pathological examination of the mice, it was found that the experimental subjects mainly died from nerve infections caused by pathogens.
It appears that the 'Sutherland bacteria' should not be used indiscriminately; the unrestrained abuse of extremely large doses will lead to unpredictable and serious consequences for the host.
Next, we begin to calculate the upper limit of human tolerance. The mice showed symptoms on day 9 and were injected with 153 Sutherland colonies. Based on the tolerance ratio between humans and mice, 153 divided by 37.5 (taking the midpoint of the range) is approximately equal to 4.
The mouse weighs 20 grams (0.02 kg), and the average human male weighs 75 kg. The weight ratio is 1:3750. Therefore, the tolerance level for Sutherland colonies in an average male is calculated to be 4 × 3750. This means that once the number of Sutherland colonies in the human body exceeds 4, pathological symptoms will appear.
Based on Todd's internal condition, 3400 Sutherland colonies can suppress 3 superpowers. Therefore, one Sutherland colony can suppress 13 superpowers. With a conservative downward adjustment, a 75-kilogram adult male can have approximately 10 superpowers at the same time.
Through this experiment on the tolerance of Sutherland bacteria, Todd ultimately reached the following conclusions:
A 75 kg male was used as a control group.
I. The maximum amount of Sutherland bacteria that humans can tolerate is one colony (this level can already cause pathological symptoms). If the density of the Sutherland bacterial solution is 170 cfu/ml, the maximum injection volume is 88.25 ml.
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