This situation is similar to what Shuimu originally guessed. There are actually some conflicts between the fairy chakras of different sources. If they must be combined, it is not impossible to connect them through the bond of natural energy.

During the Fourth Ninja World War, Uzumaki Naruto activated both Sage Mode and Nine-Tails Mode at the same time. Of course, this also indirectly illustrates the conflict between different sources of chakra. Without the Nine-Tails' cooperation, it would be basically impossible for Uzumaki Naruto to use two combat postures at the same time.

The abnormalities in the mysterious first-generation cells and the White Zetsu cells cultivated from them were partially reflected in Mizuki's original body and clone through some means. This is not difficult to understand.

Since transplanting the super cells with theoretically infinite lifespan, Mizuki has never used the curse seal and naturally did not notice the problem.

The first time he used Sage Mode to fight Shukaku, the One-Tailed Beast, although he used Tailed Beast Chakra and Curse-Seal Chakra at the same time, his body was in chaos and completely out of control, so it cannot be used as a reference.

As for later, I have been using Sage Mode, so I didn't compare it and didn't notice any difference. However, the clone that came into contact with the curse seal for the first time showed resistance to different chakras.

"Sage Mode not only affects the mental state when used, but also has some subtle changes to the body?"

"The cells of the Fourth Hokage cannot be obtained for the time being. When I have time, I will study the differences between Uzumaki Naruto and Karin's cells. Although I have speculated about this before, I have been too busy. Now it seems that I still need to pay attention to this issue..."

After observing for a while, the entity made some adjustments to the future research direction.

The clone, who was in unbearable pain, said unhappily: "Don't worry about Uzumaki Naruto now, quickly cast the Evil Sealing Seal, we can't hold on any longer."

"Yes." Shuimu nodded and asked the clone to step forward to cast the spell, "Although we have some resistance, we are not like White Zetsu who can prevent the invasion of the curse seal."

"Of course, how can a knockoff product be the same as the genuine product? A little resistance will only make people suffer more. It is better for it to take effect and stabilize quickly."

The seal for sealing evil was planted on the right arm. The clone, who possessed powerful control tools, slowly began to control the spread of the cursed chakra and natural energy in the body.

As the cursed chakra slowly spread throughout the body, the alienation effect it brought about slowly began to manifest itself.

"That's all the change?"

The second stage of the changes in the cursed seals of the Oton Four is really amazing. Not to mention the improvement of Kimimaro's physical fitness and the strengthening of the bloodline limit of the Shikotsuki to an extremely terrifying level. Even Jirobo's strange strength is already enviable, and there is also Kidomaru with many arms. The blessing of so many arms on combat power is also considerable. Others have also made good progress.

The most surprising thing is Uchiha Sasuke. The increase in chakra and the strengthening of the Sharingan are nothing but the flying ability brought by his giant flesh-winged hands that are like wings is so enviable.

On the other hand, Shuimu didn't see any special changes except that his body became a little taller, his strength and speed increased a little, and there were protrusions similar to horns behind his head. His pair of red eyes seemed to bring good observation and night vision capabilities, but there were basically no other benefits.

"The change is too small. We can't blame the curse for not being strong. We can only say that our potential is just like this."

If we don't consider the side effects of curse seals, the stronger the strength and the greater the potential, the more obvious the effect. For mediocre people with insufficient potential, not only will the effect be poor, but even their tolerance will be limited. It's no wonder that the survival rate of those who have curse seals is not high.

"This is the result after the super cells were transplanted. It is really disappointing."

"That's it. A villain that has a bloody collapse in the first episode, do you think he has any great potential?"

After the Heaven's Curse Seal was completely stabilized, the clone tested the strength of his body.

"At this level, if it was the original Mizuki, he would barely be at the level of a special jonin."

"It's as expected. This is almost the limit for civilian ninjas." Mizuki carefully checked the clone's body. There seemed to be nothing abnormal, so he ordered, "Start channeling the chakra of the One-Tail Shukaku..."

"clear!"

As soon as he finished speaking, the seal somewhere in the clone's body was instantly broken, and a trace of tailed beast chakra began to flow in the body, colliding head-on with the natural energy raging in the body.

"This feeling is so difficult to control. The tailed beast chakra cannot be driven at all..."

In a moment, the force of the explosion far exceeded expectations.

"Don't worry about the tailed beast's chakra, let it develop freely, you are only responsible for controlling the Heaven's Curse Seal."

If the cursed chakra could be slightly affected, the tailed beast chakra was too overbearing, and it was extremely difficult for the clone to guide it.

The curse seal was in the explosive state of the half-tailed beast, and the double pressure made the clone feel too much pressure to bear.

"No, I can't hold it back any longer."

The chakra that carries the will of the clone is just ordinary chakra, so there are some things that cannot be done.

"Not good, the Tailed Beast's chakra is eroding the Four Symbols Seal and is beginning to seep inside."

“Stop it!”

Shuimu formed seals with both hands, and the barrier that had been set up long ago began to work, isolating the clone from contact with natural energy. After a while, the red chakra that had been constantly flowing out gradually subsided and disappeared into the clone's body. Then the clone controlled the seal to gradually lift it, and after a long time, the changes were completely calmed down.

"How do you feel?"

Looking at the clone sitting cross-legged on the ground gasping for breath, Shuimu went forward to check and did not find any major injuries.

"The chakra of the tailed beasts that invaded the Four Symbols Seal is not enough. If it were any later, I would have died once more."

The corrosive effect of the tailed beast chakra is stronger than the curse seal, and the sealing characteristics of the one-tail chakra make it easy to break free from the seal. There is also the Four Symbols Seal, which has a good internal sealing effect, but is much less effective against external invasions.

"It's definitely not going to work this way. The final result will most likely be that the tailed beast will be resurrected in the body, and the clone will become a sacrifice for its present life."

"The chakra quality of ordinary shadow clones is insufficient, and the quantity is useless. Of course, I can activate the Sage Mode and use the shadow clones to create fake Jinchūriki, but it is extremely difficult to replenish the chakra of Sage Techniques..."

The clone cannot maintain or replenish the chakra of the immortal technique, and can only rely on the main body to handle it, which greatly goes against his original intention...